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Sunday, December 6, 2020 | History

2 edition of Electron microscopic and immunochemical studies on the small intestine of newborn piglets. found in the catalog.

Electron microscopic and immunochemical studies on the small intestine of newborn piglets.

Artur G. M. Mattisson

Electron microscopic and immunochemical studies on the small intestine of newborn piglets.

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Published by Almqvist & Wiksell in Stockholm, Göteborg [etc.] .
Written in English

    Subjects:
  • Intestine, Small.,
  • Immunochemistry.,
  • Piglets -- Anatomy.

  • Edition Notes

    Bibliography: p. 587-589.

    Statement[By] Arthur G. M. Mattisson [and] Börje W. Karlsson.
    SeriesArkiv för zoologi,, ser. 2, bd. 18, nr. 26.
    ContributionsKarisson, Börje W., 1934- joint author.
    Classifications
    LC ClassificationsQL1 .A55 ser.2. bd. 18, nr. 26
    The Physical Object
    Pagination575-589 p.
    Number of Pages589
    ID Numbers
    Open LibraryOL5655711M
    LC Control Number68098642

    The development of gastrointestinal motor activity in newborn piglets - the role of ghrelin and obestatin. Head of the project, (PLN). - National Science Centre Poland no. /03/D/NZ9/ The role of obestatin in the small intestinal development in the early postnatal period. Principal Investigator, (PLN). immunoelectron microscopy: [im′yənō′ilek′tron] electron microscopy of specimens labeled with antibodies that have been conjugated with gold. The gold makes the antibody labels electron-dense. Porcine epidemic diarrhea virus (PEDV) is having a severe effect on the pig breeding industry in central China. The mucosa and the content of the small intestine from newborn pre-weaned piglets with diarrhea were tested for the presence of PEDV by molecular and morphologic methods, and found to be positive. Negative-staining electron microscopy (EM) revealed the presence of coronavirus-like.


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Electron microscopic and immunochemical studies on the small intestine of newborn piglets. by Artur G. M. Mattisson Download PDF EPUB FB2

Leblond, C. P., and Messier, B.: Renewal of Chief Cells and Goblet Cells in Small Intestine as Shown by Radioautography after Injection of Thymidine-H3 into Mice, Anat Rec ((Nov)) Cited by:   Preliminary observations indicate that scanning electron microscopy is a useful method for studying the surface of the small intestine.

Studies of Small-intestinal Mucosa with the Scanning Electron Microscope | The BMJCited by: Abstract An electron microscopic study of intestinal epithelial cells of neonatal piglets infected with transmissible gastroenteritis (TGE) virus revealed a unique parasite-host cell by: The present study attempted to investigate the intestinal mucosa structure changes in the postnatal pig using light and scanning electron microscopy technique.

Experiments were carried out on sow. ULTRASTRUCTURE RESEA () An Electron Electron microscopic and immunochemical studies on the small intestine of newborn piglets.

book Study of the Structure of the Small Intestine in Whipple's Disease1'~ ALAN S. COHEN The Robert Dawson Evans Department of Clinical Research, Massachusetts Memorial Hospitals, and the Department of Medicine, Boston University School of Medicine, Boston University Medical Center Received Aug Cited by: The small intestine is approximately –3 cm in diameter, and is divided into three sections: The duodenum is the first section of the small intestine and is the shortest part of the small intestine.

It is where most chemical digestion using enzymes takes place. The jejunum is the middle section of the small intestine. Gross and Microscopic Anatomy of the Small Intestine. The small intestine is the longest section of the digestive tube and consists of three segments forming a passage from the pylorus to the large intestine: Duodenum: a short section that receives secretions from the pancreas and liver via the pancreatic and common bile ducts.

The regional and topographic distribution of endocrine cells in the human intestine was examined by immunohistochemistry. The frequency of endocrine cells was greatest in the small intestine with the rectum next in order.

The duodenum and jejunum harbored a large number of different endocrine cell types; the spectrum of cell types gradually narrowed distally in the intestine. Immunoproliferative small intestinal disease, also known as alpha chain disease, is a mucosa-associated lymphoid-tissue (MALT) lymphoma characterized by infiltration of the bowel wall with a.

The small intestine is an organ located in the gastrointestinal tract, between the stomach and the large intestine. It is on average 23ft long and is comprised of three structural parts; the duodenum, jejenum and ileum.

Functionally, the small intestine is chiefly involved in the digestion and absorption of nutrients. It receives pancreatic secretions and bile through the hepatopancreatic duct. Kazuyuki Ono and Minoru Takashio, Scanning electron microscopic studies of ileal epithelial cells in suckling rats, Anatomy and Embryology, /BF,1, (), ().

Crossref I.T. Johnson and J.R. Bronk, The ultrastructure of rat intestinal epithelium as revealed by cryoultramicrotomy and transmission electron microscopy. under an electron microscope at a magnification ofDFA.

Cryostat sections of small intestine and colon were tested for BCV by DFA as previously described Cryostat sections (4–6 mm thick) of fresh tissues were mounted on glass slides. Sections were fixed with acetone and stained with fluorescein conjugate (DVL BDV)a against.

The scanning electron microscope is capable of 25 mμ resolution combined with a great depth of focus, features which make the technique of value in the study of the intestinal mucosa. Surgical biopsies and postmortem specimens of small intestine have been examined using scanning and transmission electron microscopy.

Small intestine (Muscularis Externa) Webscope Imagescope Study the orientation of the smooth muscle cells in the intestinal muscularis externa. The micrograph will help you understand the pattern, which arises from the inner circular layer and outer longitudinal layer of smooth muscle cells.

The small intestine of piglets orally infected with rotavirus was examined by electron microsc 24, 48 and 60 hours after infection. At 18 and 24 hours after infection columnar epithelial. Ever since electron microscopy became an important tool in the scientific research, the focus had been mainly on ultrastructural analysis with little success in the development and application of suitable techniques for the localization of macromolecules in cells.

The emergence of immunogold techniques in the s managed to fill this gap in serving this function. SUMMARY The small intestine of naturally reared piglets ranging in age from 0 to 72 h was examined by the immunofluorescent technique and electron microscopy to study the uptake and cessation of uptake of colostral immunoglobulins by the small intestinal epithelium.

ning and transmission electron microscopic changes in the small intestine of newborn gnotobiotic puppies in- fected with a canine rotavirus isolated from a neonatal puppy with fatal diarrhea.9 This canine rotavirus iso- late, LSU 79C, has been shown to cause clinical disease, small intestinal lesions, serum antibody re.

THE mucosa of the small intestine is intimately concerned with digestive processes and it rapidly destroys itself after the death of the organism which it serves. In studies of the histopathology of human intestinal mucosa this rapid post- ' brush' border by electron microscopy has revealedthatit is composedofrowsoffine rod-like.

The small intestine from the ligament of Treitz to colon was removed via a midline laparotomy, then immediately placed on an ice-cool metal surgical pan and flushed with 50 ml ice-cold % NaCl. Intestinal O 2 uptake was lower for piglets receiving TPN relative to ENT, an immunohistochemical and immunochemical study.

Abstract. At birth the porcine intestinal barrier to macromolecular protein is minimal but is rapidly established after feeding colostrum. Macromolecular protein absorption induces ultra-structural changes in duodenal 10 and jejunal 8,9 cells of the newborn pig.

The neonatal intestinal epithelial barrier to intraluminal microorganisms has not been defined. Light and electron microscopy of cells in pig colostrum milk and involution secretion. Longitudinal study of lymphocyte subsets and major histocompatibility complex-class II expressing cells in mammary glands of sows.

The duration of colostral immunoglobulin uptake by the epithelium of the small intestine of neonatal piglets. Rotavirus particles are easily visualized by electron microscopy techniques (negative staining (Figure 4) and immune electron microscopy), considering that they are present in large quantities in feces and intestinal fragments of infected pigs.

These techniques have been used in many studies of the swine rotavirus [ 46 – 48 ]. Figure 4. 24 Small intestine, structure of intestinal plica and villi 32 Small intestine, Ileum, villi and lacteal - The third level of folding is observed in the apical surface of epithelial absorptive lining cells (enterocytes), they are know as the microvilli.

At the optical microscope the microvilli appear as a continuous band in the apical part of. Start studying 3-E: microscopic structure of the small intestine.

Learn vocabulary, terms, and more with flashcards, games, and other study tools. An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination.

As the wavelength of an electron can be up totimes shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.

A scanning transmission electron microscope has achieved. Intestinal and colonic biopsy specimens were ob- tained as a routine part of the clinical evaluation of 6 patients with established AIDS and 2 patients with ARLS.

Five small intestinal biopsy specimens and six rectal biopsy specimens from 8 patients were available for light- and electron-microscopic study.

This study was designed to evaluate liver disease in neonatal piglets with surgical short bowel syndrome causing intestinal failure with partial parenteral nutrition dependence.

The short bowel piglets had 75% surgical resection of distal small intestine, including all ileum and cecum, and were compared with sham controls, without resection.

Scanning electron microscopic observations on the intestinal villi in growing white leghorn and broiler chickens from 1 to 30 days of age.

British Poultry Science: Vol. 32, No. 1, pp. Yamamoto, T.: An electron microscopic study of the columnar epithelial cell in the intestine of fresh water teleosts: Goldfish (Carassius auratus) and rainbow trout (Salmo irideus).

Zellforsch. 72, 66–87 (). Google Scholar. Electron microscopy is of particular value for peripheral nerve sheath tumours, marker‐negative synovial sarcomas, pleomorphic sarcomas and mesotheliomas.

As with all adjunctive techniques, immunohistochemistry and electron microscopy should be used in a complementary fashion according to the nature of the diagnostic problem.

The topological architecture of the entire intestinal tract of the snow trout, Schizothorax curvifrons has been investigated with the aid of great field of depth and resolving power of Scanning Electron Microscope (SEM) after carrying out their primary fixation in % glutaraldehyde in M sodiumcacodylate buffer (pH ) for 24 h at °C and secondary fixation in % osmiumtetraoxide.

The small intestine is divided into three distinct segments, from oral to aboral: duodenum, jejunum, and ileum. The major functions of the small intestine are digestion, secretion, and absorption.

The small intestinal mucosa has several anatomic adaptations that serve to create an immense surface area with which to digest and absorb nutrients. Immunochemical Methods.

Immunocytochemistry or immunohistochemistry is the identification of a tissue constituent (protein, lipopolysaccharide, etc.) in situ by means of a specific antigen/antibody reaction tagged by a visible label. immunohistochemistry usually refers to a light level detection and immunocytochemistry usually refers to detection at EM resolution.

The present study attempted to investigate the intestinal mucosa structure changes in the postnatal pig using light and scanning electron microscopy technique. Experiments were carried out on sow reared piglets from birth until 38 days of age.

Piglets were sacrificed. CHN-JS infection-induced microscopic lesions were observed, and viral antigens were detected mainly by immunohistochemical staining in the small intestine. The neonatal Fc receptor (FcRn) and polymeric immunoglobulin receptor (pIgR) are crucial immunoglobulin (Ig) receptors for the transcytosis ofimmunoglobulin G (IgG), IgA, or IgM.

This study aims to investigate the effects of exogenous catalase (CAT), an antioxidative enzyme from microbial cultures, on intestinal development and microbiota in weaned piglets. Seventy-two weaned piglets were allotted to two groups and fed a basal diet or a basal diet containing g/kg exogenous CAT.

Results showed that exogenous CAT increased (p < ) jejunal villus height/crypt. When killed 72 h after infection, intestinal villi were shorter and crypts deeper (P less than ) in duodenum, upper jejunum, and mid-small intestine, but not ileum in infected piglets.

Virus antigen was seen by fluorescence microscopy in occasional jejunal villus tip cells in only four infected piglets and no controls at 72 h. Pigs are ungulate animals of the genus Suis and family Suidae. They are globally spread but restricted in certain countries due to religious and cultural beliefs.

Pork serves as an important source of protein (38% of meat consumed in the world). While pig production remains a profitable enterprise, commercial and particularly the small-scale farmers face huge constraint in this husbandry.

Scanning electron microscopy of intestinal villous structures and their putative relation to digestion and absorption in chickens. ESMAIL Department of Animal Science, Weber Hall, Kansas State University, Manhattan, KS, U.S.A. Summary. Scanning Electron Microscope (SEM) was used to examine villous struc- tures at different parts in the small intestines of week-old chickens.

Gross and Microscopic Anatomy of the Large Intestine. The large intestine is that part of the digestive tube between the terminal ileum and anus. Depending on the species, ingesta from the small intestine enters the large intestine through either the ileocecal or ileocolic valve.

Within the large intestine, three major segments are recognized. Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry.

This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened.Intestinal Mucosa Subject Areas on Research.